Detaylı ürün açıklaması
KOVACS′ indole reagent
for detecting microbial indole in the identification of indole-positive and indole-negative microorganisms, suitable for microbiology
Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.
form
liquid
packaging
pkg of 100 mL
technique(s)
microbe id | specific enzyme detection: suitable
color
yellow
pH
<1 (20 °C in H2O)
transition temp
flash point 36 °C
density
0.92 g/cm3 at 20 °C
application(s)
microbiology
storage temp.
2-8°C
suitability
Escherichia coli, enterovirulent E. coli (EEC) spp.
